Abstract: In order to develop black chokeberry juice beverage with healthy function and investigate its antioxidant activity, with the black chokeberry fruit as the main raw material, sucrose, citric acid and β- cyclodextrin were added, and sensory scores as the index. By single factor experiments and orthogonal experiment, the optimum formula of black chokeberry fruit juice beverage was determined as follows: The dosage of chokeberry extract juice was 60%, the dosage of 60% sucrose syrup was 10%, the dosage of 2% citric acid solution was 2.0%, and the dosage of 1% β-cyclodextrin was 3.0%. The sensory scores of the beverage prepared by this formula could reach 94.5 scores. The beverage prepared by the formula was rich in 16 kinds of amino acids, with a total amount of 374.79 μg/mL, it had the characteristic aroma of black chokeberry fruit, with bright and uniform color, harmonious and refreshing taste, and uniform texture. The results of antioxidant activity in vitro showed that the prepared black chokeberry beverage had good antioxidant capacity on DPPH radical scavenging rate, hydroxyl radical scavenging rate, ABTS⁺ radical scavenging capacity and total reducing capacity, which reached 36.42%, 32.58%, 0.54 mmol/L and 0.289, respectively. The results of this experiment would provide a theoretical basis for the development of black chokeberry functional beverage.