Abstract: In order to explore the high-value utilization of mango peel waste, mango peel extract (MPE) was used by microwave technology. Chitosan (CS) was dissolved in glacial acetic acid (AAG) or citric acid (CA), then MPE was blended with polyvinyl alcohol (PVA) and CS. The PVA/CS/MPE composite film was prepared by solution casting. The structure, morphology and thermal properties of the composite film were characterized by UV, FT-IR, SEM and TG to determined the mechanical properties, water resistance, antibacterial, anti-oxidation and degradation properties of the composite film. The experimental results showed that: Compared with ordinary stirring (35 °C, 500 r/min, 4 h, yield 13.86%), the yield of MPE under the microwave process (35 °C, 300 r/min, 1 h) increased to 38.26%. The components of the composite film were mainly combined through hydrogen bonding with good compatibility and without obvious phase separation. The composite film showed good antibacterial activity and degradability. The elongation at break of the composite film of the CA system was nearly double that of the pure PVA film, and the elongation at break was higher after adding MPE. MPE could significantly improve the water resistance of the composite film in AAG system. Meanwhile, MPE improved the UV resistance and oxidation resistance of the composite film. Therefore, there are the extensive prospect in medicine and food packaging about the PVA/CS/MPE composite film.