Abstract: Objective: To explore the protective effect of deer skin gum polysaccharide on acute alcoholic liver injury. Methods: The crude deer skin gum polysaccharide was extracted by extraction method, and purified by column chromatography to obtain pure deer skin gum polysaccharide, and its physical and chemical properties were identified by column chromatography, infrared spectroscopy and physical and chemical reaction experiments. The content of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) in mouse serum and the activity of GSH-Px, SOD and the content of MDA in the supernatant of liver homogenate were measured. The liver tissue sections of mice were observed to explore the protective effect of deer skin gum polysaccharide on acute alcoholic liver injury. Results: The deer skin gum polysaccharide was an acid polysaccharide with the pyranose structure. Its shape was white flocculent, with a molecular weight of 41.2 kDa and the purity of 86.71%. It had no starch, phenols, protein, amino acids and reducing sugar residues.The animal experiment results showed that compared with the model group, the deer skin gum polysaccharide middle and high dose groups could significantly increase the SOD and GSH-Px activities in the serum of mice (P<0.05), and reduce the ALT, AST activities and MDA content (P<0.05). At the same time, it could significantly increase the activity of SOD and GSH-Px in the supernatant of liver tissue homogenate (P<0.05), and significantly reduce the content of MDA (P<0.05). Conclusion: Deer skin gum polysaccharide has obvious protective effect on mice with acute alcoholic liver injury.