Abstract: This study investigated the protective effect of curcumin/cyclodextrin polymer inclusion complex(CUR/CDP) on LO2 cells damaged by ethanol. The MTT method was used to establish a model of LO2 cells damaged by ethanol. Glutamate alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH), superoxide dismutase(SOD), glutathione peroxidase(GSH-PX), malondialdehyde(MDA) and reactive oxygen species(ROS) kits were used to investigate the protective effect of CUR/CDP on LO2 cells damaged by ethanol. The results showed that after CUR/CDP (80 μg/mL) treatment, the cell activity of LO2 cells increased from (54.75%±8.97%) (model group) to 85.27%±2.64%, and the activities of ALT, AST, and LDH in the LO2 cell culture medium decreased from (26.47±0.90), (41.02±4.41), (63.77±4.95) U/L (model group) to (16.17±0.42), (22.62±0.79), (32.25±1.69) U/L (P<0.01), respectively; the activities of GSH-PX and SOD in LO2 cells increased from (21.82±1.34), (8.45±1.11) U/mg prot (model group) to (36.70±0.56), (16.47±1.27) U/mg prot; the content of MDA and ROS in LO2 cells decreased from (1.19±0.15) nmol/mg prot, (198.02%±11.76%) (model group) to (0.72±0.05) nmol/mg prot, (110.87%±10.22%) (P<0.01), respectively. In conclusion, CUR/CDP could improve the damage of LO2 cells induced by ethanol by increasing the activity of antioxidant enzymes and reducing the content of ROS.