Abstract: Using dual-protein nutritional powder as basic material, high fiber dietary combination (konjac powder, Chia seed, inulin), maltodextrin and fruit powder as raw materials, a dual-protein nutritional meal substitute powder with high protein, high fiber, easy satiety, low fat content and rich nutrition was developed. The crushing methods and mixing formula of high fiber dietary combination were studied, and it was found that the ball milling products of three kinds of raw materials had high yield, light color and uniform particles. The formula of meal substitute powder was optimized by single factor and orthogonal experiments, and the best formula was obtained as follows: 74% of dual-protein nutrition powder, 12.3% of high fiber diet, 8.5% of maltodextrin and 5.2% of fruit powder. The meal substitute powder of this formula could be dissolved quickly, and was fine and smooth, as well as with rich aroma. The nutritional composition and powder characteristics of meal substitute powder were analyzed. The results showed that the protein content was significantly higher than that of similar products on the market (the protein content of 100 g dual-protein meal replacement powder was 39 g). The meal replacement powder was rich in nutrition, with good fluidity and no strong tendency to splash, and met the Chinese and International Standards requirements. This study would provide a new direction for the development of meal replacement powder industry in our country.