Abstract: A method was established for the simultaneous determination of 11 fungal toxins in coffee beans by ultra-performance liquid chromatography tandem mass spectrometry, the effects of column selection, extracting solvent composition, solid phase extraction column selection and flow phase ratio on the analysis results were examined. Taking coffee beans as the research object, the samples were extracted by acetylene-water-formic acid (85+14+1, V/V) purified by Oasis PRiME HLB, the purification diluted with phosphoric acid buffer, nitrogen blowing after the purified by multi-functional immunoaffinity column, determined by ultra-performance liquid chromatography tandem mass spectrometry after resoluble with 50% acetylene, with 0.1% foric acid-2 mmol/L ammonium acetate solution-acetylene as the flow phase, separated by Waters BEH C₁₈ column, determined by ultra-performance liquid chromatography tandem mass spectrometry quantitated by internal standard method. The results showed that, the linear correlation of 11 mycotoxins were greater than 0.998, the limits of detection were in the range of 0.008~0.544 μg/kg. The average recoveries at the three spiked levels were 80.2%~114% with relative standard deviations of 1.4%~7.9%. This method was simple, fast and efficient, and would be suitable for the co-measurement and analysis of 11 mycotoxins in coffee beans.