Abstract: In order to investigate the chemical components in Toricellia Angulata (Harms) Hu, this paper analyzed and inferred the chemical components in Toricellia Angulata (Harms) Hu based on the method of HPLC-HESI-HRMS.ThermoFisher Hypersil GOLD aQ C₁₈(100 mm×2.1 mm, 1.9 μm)column was adopted, with 0.1% formic acid- methyl cyanides(0.1% formic) as the mobile phase for gradient elution, the flow rate was 0.3 mL/min, the column temperature was 40 ℃; HESI-II ion source was used for mass spectra, the date were collected in positive and negative mode.Mass scanning range m/z: 100~1500 Da.Compound discoverer 3.0 database and coupled with the related literature were used to analyze the main chemical constituents of Toricellia Angulata (Harms) Hu.The results showed that thirty-six chemical components in Toricellia Angulata (Harms) Hu were inferred by analyzing positive and negative ion spectra information of high resolution mass spectrometer with date from relevant literature.Including 10 organic acids, 4 alkaloids, 1 amino acid, 2 phenylpropanoids, 2 esters, 2 terpenes, 2 aromatic oxygen derivatives, 4 amides, 9 other compounds.HPLC-HESI-HRMS provides the technical support for the quality control of Toricellia Angulata (Harms) Hu and contributes the reference data to elucidating the potential basis of Toricellia Angulata (Harms) Hu.