Abstract: In order to explore the comprehensive quality of introduced blueberries in Shandong Province, the morphological index, physical and chemical index and texture index of 13 kinds of blueberries were determined, and the comprehensive evaluation was made by correlation analysis and principal component analysis, and the rankings were obtained by cluster analysis. The results showed that there were differences among different varieties, and the comprehensive quality of big blue gold was the best. The fruit was round in shape and bright in color, with total sugar content of 17.07 g/kg, vitaminC content of 1.35 mg/g, SOD content of 1156.11 U/g, excellent texture indexes and good taste. The first 5 principal components were extracted from 17 indexes by principal component analysis, and the cumulative variance contribution rate was 83.260%. A mathematical model for comprehensive evaluation of blueberries was established based on principal component analysis data. The scores showed that the comprehensive qualities of big blue gold, Bladen, Herbert and Sunrise were higher. When the European distance was 5, blueberries were divided into five categories by cluster analysis. Combined with sensory evaluation results, it was found that big blue gold was the best variety. Blueberries were sweet and sour, rich in nutritional value, and had high economic value and broad development prospects. The comprehensive quality evaluation of introduced blueberries in Shandong Province provided theoretical basis for comprehensive utilization and deep processing of blueberries in Shandong region.