Abstract: In the study, the extraction conditions and antibacterial activities of polysaccharides from Meitan white tea were studied. The effects of extraction temperature, extraction time and solid-liquid ratio on the polysaccharide yield were investigated. The optimum extraction parameters were determined by orthogonal test, which the extraction temperature was 90 ℃ for 4 h, and solid-liquid ratio 1:20 g/mL. Under these conditions, the polysaccharide yield of Meitan white tea was 1.163%±0.011%. The antibacterial activity test showed that Meitan white tea polysaccharide had inhibitory effects on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans, which the inhibitory activity was the strongest against Staphylococcus aureus, MIC was 3.125 mg/mL. The research results provide theoretical guidance for further research and new product development of Meitan white tea polysaccharide.