Abstract: A gas chromatography-mass spectrometry (GC-MS-MS) method was established for the detection of 9, 10-anthraquinone in tea packaging paper. The sample was extracted with acetone-n-hexane mixed solution, and then the extract was concentrated by centrifugation and nitrogen blowing, and then purified by SPE with a Florisil solid phase extraction column. The purified liquid was analyzed by a gas-mass spectrometer. The instrument used DB-5MS gas chromatography. Column (20 m×0.18 mm×0.18 μm) was separated, scanning in multi-reaction monitoring mode was selected, and internal standard method was used for quantification. The results showed that the method had a good linear relationship in the mass concentration range of 10~200 ng/mL, the linear regression equation was Y=0.23249X+0.001203, the fit R² was 0.9989, the detection limit of the method was 3 μg/kg, the limit of quantification was 10 μg/kg, the recovery rate of standard addition was 89.3%~98.7%, and the relative standard deviation (RSD) was 3.01%~4.42%. This method was suitable for the qualitative and quantitative detection of 9, 10-anthraquinone in tea packaging paper.