Abstract: The polysaccharide based blend films have the characteristics of uniformity, transparency and degradability. It has become a hot research direction in the field of biodegradable film. Studies have found that mixing nanoparticles with polysaccharide solution could form a complex film-forming solution. After film formation, the original microstructure of the film changed, thereby improving the mechanical and barrier properties of the blend film. In this paper, the microstructure and molecular interaction in starch film, chitosan film and konjac glucomannan film were reviewed. The changes in microstructure and physicochemical properties of the films blended with nanoparticles and polysaccharides were emphatically described, in order to provide reference for the microstructure design and film performance control of the polysaccharide based blend films.