Abstract: Polysaccharides is a kind of macromolecule polymer which the widely exists in life,some of which have biological activities such as improving immunity,antioxidant,anticoagulant,anticancer,antiviral,hypoglycemic,etc. Therefore,active polysaccharides play an important role in food,medical treatment,chemical industry and many other fields. Compared with other extraction methods,enzymatic extraction has the advantages of mild reaction conditions,high extraction efficiency,high product activity,low cost,energy saving and environmental protection. In this paper,the research progress of enzymatic extraction of polysaccharides in recent years was reviewed,including the principle and influencing factors of enzymatic extraction of polysaccharides,single and multiple enzyme extraction,the combination other technologies with enzymatic extraction technology,and the advantages of enzymatic extraction of polysaccharides. The application prospect of this method is prospected.