数字PCR技术在食品检测中的应用研究进展

刘可; 李梦霞; 张亮; 康大成; 扈晓杰; 刘云国

doi:10.13386/j.issn1002-0306.2020040194

数字PCR技术在食品检测中的应用研究进展

刘可^1,2,
李梦霞³,
张亮⁴,
康大成²,
扈晓杰²,
刘云国^2, ,

1. 新疆大学生命科学与技术学院, 新疆乌鲁木齐 830046;
2. 临沂大学生命科学学院, 山东临沂 276000;
3. 东营区科学技术局, 山东东营 257000;
4. 临沂小鲁生物科技有限公司, 山东临沂 276000

基金项目:

2019年山东省重点研发计划（医用食品专项计划）（2019YYSP026）。

详细信息

作者简介:
刘可(1995-),女,硕士研究生,研究方向:食品微生物,E-mail:liuke95625@163.com。

通讯作者:
刘云国(1977-),男,博士,教授,研究方向:食品微生物,E-mail:yguoliu@163.com。

中图分类号: TS207.3
计量
- 文章访问数: 552
- HTML全文浏览量: 25
- PDF下载量: 61
出版历程
- 收稿日期: 2020-04-16
- 网络出版日期: 2021-03-01
- 刊出日期: 2021-02-14

Research Progress of Digital PCR Technology and Its Application in Food Detection

1. School of Food Science and Technology, Xinjiang University, Urumqi 830046, China;
2. School of Life Sciences, Linyi University, Linyi 276000, China;
3. Dongying District Bureau of Science and Technology, Dongying 257000, China;
4. Linyi Xiaolu Biotechnology Co., Ltd., Linyi 276000, China

摘要

摘要: 数字PCR是近年来迅速发展的一种核酸分子定量检测技术，与传统检测方法相比具有不依赖标准曲线、灵敏度和精准度高等优势，在食品转基因成分分析、动植物产品检测、微生物检测等相关领域逐渐显现出巨大的应用价值和发展前景。本文对数字PCR的原理、应用、存在的问题及发展趋势等进行综述及分析，为数字PCR在食品检测中的应用提供参考。
- 数字PCR /
- 转基因产品 /
- 食品源性成分 /
- 微生物检测
Abstract: Digital PCR(dPCR)technology is a rapid development of absolute quantification of nucleic acid molecules in recent years. It has the advantages of quantification without standard curve,high sensitivity and high accuracy. With the increasing requirements of detection technology in various fields,the application of digital PCR technology in the analysis of genetically modified products,animal and plants products,microorganisms and other fields has become more and more widespread. This article reviews the principles and applications of dPCR,analyzes its problems and prospects,and provides a reference for the application of digital PCR.
- digital PCR /
- genetically modified products /
- derived ingredients /
- microorganism

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