Abstract: The traceability of stable isotopes is to use the differences of natural abundance of stable isotopes in animals and plants in different regions to identify the animals and plants environment. In recent years, it has been successfully used in food adulteration and food origin verification, and has shown unique advantages. Based on the research results of food traceability at home and abroad, this paper reviews the application and progress of stable isotope technology in animal and plant food traceability, analyzes the difficulty and development direction of isotope traceability technology, which can provide theoretical basis for the perfection of food safety traceability system.