Preventive Effect of Walnut Oligopeptide on Alcoholic Gastric Ulcer in Rats
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摘要: 目的：本研究通过建立无水乙醇诱发的大鼠急性胃溃疡模型，探讨核桃低聚肽（Walnut oligopeptides，WOPs）对酒精性胃溃疡的保护作用及其可能机制。方法：雄性SD大鼠随机分为7个剂量组（n=10）：正常对照组、模型对照组、奥美拉唑对照组（20 mg/kg）、WOPs低、中、高剂量组（220、440、880 mg/kg）。灌胃干预30 d后，采用无水乙醇5 mL/kg灌胃造模，然后进行胃溃疡指数评分，血液学参数检测及胃组织中前列腺素E2（Prostaglandin E2，PGE2）、一氧化氮（Nitric oxide，NO）、髓过氧化物酶（Myeloperoxidase，MPO）含量的测定。结果：WOPs低、中、高剂量组胃溃疡指数显著低于模型对照组（P<0.05），其中WOPs高剂量组胃溃疡指数显著低于奥美拉唑组（P<0.05）；WOPs对模型大鼠白细胞计数、单核细胞百分数的异常升高具有显著的抑制作用（P<0.05）；WOPs还可以显著提高胃组织PGE2含量，降低MPO、NO水平（P<0.05）。结论：WOPs对酒精性胃溃疡具有较好的防护作用，其主要作用机制与降低胃溃疡严重程度，增加PGE2含量，减少中性粒细胞的聚集浸润和一氧化氮含量病理性升高有关。Abstract: Objective:This study explored the protective effect of walnut oligopeptides(WOPs)on alcoholic gastric ulcer and its possible mechanism by establishing a model of acute ethanol-induced gastric ulcer in rats. Methods:Male SD rats were randomly divided into 7 dose groups(n=10):Normal control group,model control group,omeprazole control group(20 mg/kg),WOPs low,medium and high dose groups(220,440,880 mg/kg,respectively). After oral gavage for 30 consecutive days,rats were given 5 mL/kg absolute ethanol to induce gastric ulcer. Then the levels of gastric ulcer index,hematological parameters,prostaglandin E2(PGE2),nitric oxide(NO)and myeloperoxidase(MPO)of gastric tissues in rats were evaluated. Results:The gastric ulcer index of the WOPs low,medium and high dose groups was significantly lower than that of the model control group(P<0.05),and the gastric ulcer index of the WOPs high dose group was lower than that of the omeprazole group(P<0.05). WOPs had a significant inhibitory effect on the abnormal increase of white blood cell count and monocyte percentage in model rats(P<0.05). In addition,WOPs could significantly increase the content of PGE2 and reduce the levels of MPO and NO(P<0.05). Conclusion:The WOPs would have protective effect on alcoholic gastric ulcer,and the probably mechanism of its action was related to reducing the severity of gastric ulcer,increasing the contents of PGE2,reducing the aggregation and infiltration of neutrophils and the pathological increasing of nitric oxide contents.
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Keywords:
- walnut oligopeptides /
- absolute ethanol /
- gastric ulcer /
- prostaglandin E2
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