Abstract: To investigate the effect of slightly acidic electrolyzed water (SAEW) treatment on the quality change of wild fungus during storage, the Box-Behnken experiment was designed with response surface method, while SAEW processing time, processing temperature and solid-liquid ratio were as factors, and total number of colonies of fresh wild fungus surface for the death of the order of magnitude and sensory score were as response value. The fresh wild fungus was treated with the optimized SAEW treatment conditions and put in 4℃ for storage, and the total number of colonies, malondialdehyde content, polyphenol oxidase activity and weightlessness rate and vitamin C content were determined. Results showed that the best processing conditions for SAEW were:Processing time 5 min, processing temperature of 20℃, and solid-liquid ratio 1:15 g/mL. Under the optimized conditions, the growth of microorganisms on the surface treated with SAEW was inhibited effectively, the process of storage of vitamin C content and the increase speed of malondialdehyde content was delayed, the activity of polyphenol oxidase was inhibited, the weightlessness rate had no significant effect. The total number of bacterial colonies in the storage process was only 1.83 lg CFU/g. Compared with the control group, the content of malonaldehyde decreased by 0.434 μmol/L, the activity of polyphenol oxidase decreased by 0.49 U and the weight loss rate was 1.27%. Comprehensive showed that SAEW treatment on fresh wild fungus not only could control microbial growth, but also could slow down the speed of storage quality deterioration. The results would provide SAEW in the use of wild fungus fresh-keeping technology to provide theoretical basis.