Abstract: Seabuckthorn seed oil is a functional oil rich in unsaturated fatty acids,and therefore highly susceptible to oxidation. This article combined the addition of natural antioxidants with microcapsule preparation technology,which could resist the oxidation. Firstly,the composition of seabuckthorn seed oil was determined,then seabuckthorn seed oil was used as the core material,and the selected natural antioxidant L-AP was added,and sodium starch octenyl succinate,sodium caseinate and maltodextrin were used for encapsulation by spray drying. Seabuckthorn oil microcapsules were prepared from the materials,and the oxidation stability was evaluated by an accelerated storage experiment in an oven at 65 ℃. Results showed that sea buckthorn seed oil had high unsaturated fatty acids contents 80.6%±2.10%. Ultimately,microcapsules contained 15% OSA-modified starch,5% casein,5% maltodextrin,30% resistant malt dextrin,10% sugarcane juice concentrate,30% sea buckthorn seed oil,5% isomaltulose and 3 g/L sodium tripolyphosphate. The encapsulation efficiency of microcapsules was as high as 91.8%±1.59%. Sea buckthorn seed oil were placed inside a thermostated chamber at 65 ℃ showed that microcapsules with antioxidants could effectively improve the oxidation stability of sea buckthorn seed oil.