Abstract: In this paper,rapeseed protein was pretreated with high pressure(200,400 and 600 MPa)and heat(60,80 and 100℃)and hydrolyzed with alkaline protease(enzyme concentration:1%,3%,and 5%). The effects of different treatment conditions on rapeseed protein hydrolyzation degree,ACE,renin inhibitory activity and antioxidant properties were analyzed.The results showed that high pressure and heat treatment could reduce the hydrolysis degree of rapeseed protein.Compared with the control group,high pressure and heat treatment increased the ACE and renin inhibition activity of rapeseed protein hydrolysate. Among them,the ACE inhibition rate of rapeseed protein treated with 400 MPa increased by 10.27% at 5% enzyme concentration,and the renin inhibition rate of rapeseed protein treated with 80℃ increased by 50.06% at 5% enzyme concentration.The adsorption capacity of rapeseed protein at 1% enzyme concentration significantly reduced by high pressure or heat treatment.The Fe²⁺ chelating ability of rapeseed protein under high pressure or heat treatment was significantly enhanced at different enzyme concentrations(P<0.05).It was believed that high pressure and heat treatment could improve the biological activity of rapeseed protein to some extent.