摘要:
目的:研究方格星虫纤溶酶(SNFE)抗血栓作用。方法:体外采用比浊法测定SNFE对花生四烯酸(AA)诱导下的血小板5 min内最大聚集率(PAGM)的影响;小鼠随机分为空白组、模型组、阿司匹林组(10 mg/kg)、SNFE低剂量组(4000 IU/kg)、SNFE高剂量组(8000 IU/kg),每天进行一次灌胃给药,每7 d记录体重。给药第12 d,除空白组外,皮下注射0.8%角叉菜胶诱发尾部血栓,继续给药2 d,记录造模后48 h黑尾发生率及黑尾相对长度;末次药后2 h眼球采血及凝固法测定血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)及纤维蛋白原(FIB)。结果:SNFE低、高剂量组AA诱导下的血小板5 min内最大聚集率(PAGM)均低于空白组,抑制率分别为20.2%±4.5%和45.1%±2.5%,但与空白组比较,仅高剂量组PAGM的差异有统计学意义(P<0.01)。注射角叉菜胶48 h后,90%模型组和SNFE低剂量组小鼠出现黑尾,60% SNFE高剂量组小鼠出现黑尾;低、高剂量组的黑尾相对长度均比模型组短,但只有高剂量组有统计学意义(P<0.05)。与空白组比较,模型组小鼠的PT、APTT、TT缩短(P<0.05),FIB无明显差异;SNFE高、低剂量组的PT、APTT、TT在数值上均比模型组增加,FIB含量减少,但仅高剂量组的APTT、TT的延长有统计学意义(P<0.01或P<0.05)。结论:方格星虫纤溶酶有一定的抗血栓形成的作用。
Abstract:
Objective:The anti-thrombus effect of fibrimolytic enzyme SNFE in Sipuculus nudus Linnaeus was studied. Methods:The effect of SNFE on the maximum aggregation rate of platelet induced by arachidonic acid(AA)in 5 min was measured by turbidimetry in vitro. Mice were randomly divided into blank group,model group,aspirin group(10 mg/kg),SNFE low-dose groups(4000 IU/kg)and high-dose(8000 IU/kg). Each group was given corresponding drugs intragastrically once a day and the body weight was recorded every 7 d. On the 12th day,except the blank group,the tail-thrombus model was established by subcutaneous injection of 0.8% carrageenan. After 48 h,the incidence of thromboembolism and the relative average thrombus length in tail were calculated. 2 h after the last drug delivered,blood was collected through pricking eyeball to measure prothrombin time(PT),activated partial thromboplastin time(APTT),thrombin time(TT)and fibronectin(FIB). Results:The maximum aggregation rate of platelet in 5 min induced by AA in low and high dose group was 20.2%±4.5% and 45.1%±2.5%,respectively. And there was significant difference between the high dose group and the blank group(P<0.01). The incidence of thrombosis was 90% in model group and low dose group,60% in high dose group. The relative average thrombus length in high dose group was shorter than that in model group(P<0.05). PT,APTT,TT of model group were shortened compared with blank group(P<0.05),while there was no significant difference in FIB,and high dose group significantly prolong the activated partial thrombin time(APTT)and thrombin time(TT)(P<0.05). Conclusion:The fibrinolytic enzyme SNFE would have antithrombosis effect.
百度学术
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