Abstract: In order to screen the lactic acid bacteria(LAB)with antibacterial properties in rice pulp of Huangjiu,antibacterial LAB were isolated and purified by preliminary screening and re-screening experiments. Morphological observation,physiological and biochemical reaction were used to classify the strains. The antibacterial substances were preliminarily analyzed by excluding acid interference test. The results showed that the screened 36 lactobacllus inhibited the growth of Bacillus cereus and Escherichia coli. According to their physiological and biochemical characteristics,they were identified as L. plantarum,L. minor,L. aviarius subsp. araffinosus,L. rhamnosus,L. kefiranofaciens,L. crispatus,L. acidlophilus,etc. The antibacterial substances produced by these LAB were not lactic acid,acetic acid and hydrochloric acid. The results provided a basis for further research of antibacterial substances in rice pulp of Huangjiu.