摘要:
为系统全面的研究SFE-CO2流体萃取甜瓜蒂中葫芦素的过程,对主要控制变量进行全面分析,通过单因素实验研究携带剂用量、萃取温度、萃取压力、萃取时间、CO2流速及粒度对葫芦素萃取率的影响,并在单因素实验的基础上,采用正交试验优化SFE-CO2葫芦素的萃取工艺,得出SFE-CO2萃取葫芦素最佳条件为:携带剂用量200 mL、萃取温度35℃、萃取压力25 MPa、萃取时间30 min、CO2流速20 kg·h-1、粒度60~80目,在此条件下葫芦素的萃取率为1.443%。使用HPLC对萃取物进行分析。结果显示甜瓜蒂中含有葫芦素B、D、E和I,含量依次为7.83、1.03、3.25、2.38 mg·g-1,以葫芦素B为主,结果表明SFE-CO2萃取葫芦素可避免热敏性葫芦素的损失。
Abstract:
In order to investigate process of supercritical carbon dioxide fluid extraction(SFE-CO2)of cucurbitacin from Cucumismelo L. stem systematically and comprehensively,the main control variables were comprehensively analyzed. The effects of entrainer amount,extraction temperature,extraction pressure,extraction time,CO2 flow rate and particle size on the extraction yield of cucurbitacin were studied by single factor experiment. According to the date from the single factor experiments,the orthogonal test was used to optimize the extraction process of cucurbitacin by SFE-CO2.The best condition for SFE-CO2 extraction of cucurbitacin was as follows:The entrainer amount of 200 mL,extraction temperature 35℃,extraction pressure 25 MPa,extraction time 30 min,CO2 flow rate 20 kg·h-1,particle size 60~80 mesh. In this condition,the extraction yield of cucurbitacin was 1.443%. The extracts were analyzed by HPLC,and the results showed that cucurbitacin B was mainly composed,which contained cucurbitacin B,D,Eand I,the contents were 7.83,1.03,3.25,2.38 mg·g-1. Results showed that the SFE-CO2extraction of cucurbitacin could avoid the loss of heat-sensitive cucurbitacin.
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