Abstract: Objective:To establish a direct quantitative and qualitative detection method of Vibrio parahaemolyticus in seafood. Method:According to colony counting method of GB,the culture to count and recognize typical colony color from V.parahaemolyticus was screened. Artificially contaminated samples were tested at 50、4 and -18℃. Results:In NaCl NA double-layer agar,the number of lavender colonies from V.parahaemolyticus was no significant difference from that on NaCl NA plate in artificial bacterial contamination test. Under 4 and-18℃,the concentrations of V.parahaemolyticus of samples were 68.9% and 21.7% compared with the control group detected by NaCl NA double-layer agar direct quantitative test. Conclusion:NaCl NA double-layer agar had the advantages of simplicity,rapidity,stable result and highly sensitivity,suitable for the direct quantitative and qualitative detection of V. parahaemolyticus in seafood under-18~50℃ environment and truthfully reflecting the contamination of samples.