Abstract: In order to explore the effects of two extraction methods on the extraction of polyrphenols from Hericium erinaceus,Pleurotus eryngii and Tricholoma matsutake,and compare the antioxidant activities of three edible and medicinal Fungi extracts,the polyphenols of three edible and medicinal fungi were extracted by soaking extraction and heated reflux extraction respectively in this paper. Firstly,the polyphenol contents of these extracts were determined by the improved Folin-Ciocalteus colorimetry method of the different conditions. DPPH and ABTS tests were followed used to evaluate the ability of scavenging free radical of these extracts.In the end,the protective effect of the reflux extracts from UVB-induced photodamage of HaCaT cells was evaluated by MTT assay. Results showed that a good linear relation was exhibited between the total polyphenol contents and its absorbance and that the extraction effect of polyphenols was higher using reflux method. The polyphenol contents of reflux extract,Hericium erinaceus,Pleurotus eryngii and Tricholoma matsutake were(2.684±0.02),(4.781±0.06)and(5.028±0.05) mg/mL separately. The heated reflux extraction of Tricholoma matsutake demonstrated extremely strong scavenging effect on DPPH· and ABTS⁺· whose IC₅₀ were(0.534±0.03)and(0.271±0.01) mg/mL respectively. Furthermore,Hericium erinaceus,Pleurotus eryngii and Tricholoma matsutake increased cell viability and protected HaCaT cells against UVB-induced damages at the dose of 100 μg/mL. In summary,the study showed that the effect of extracting polyphenols in such the reflux way was better. The ability of scavenging free radicals of Tricholoma matsutake extracted by the reflux method was the strongest. However,the polyphenol contents of which was positively linked to the scavenge ability of free radicals was not related to the antioxidant capacity for cells.