Abstract: A rapid LC-MS/MS method for the determination of 5 kinds of heterocyclic amines(MeIQ,MeIQx,4,8-DiMeIQx,7,8-DiMeIQx and PhIP)in stewed meat products were established. The samples were extracted by ultrasonic extraction with 40 g/L NaOH-methanol solution and purified by polystyrene divinylbenzene solid phase extraction column(Poly-Sery PSD). The electrospray ion source positive ion(ESI⁺)and multi-reaction monitoring(MRM)mode were used for the determination of the 5 kinds of heterocyclic amines. The calibration curves of the 5 kinds of heterocyclic amines showed a good linear relationship in the ranges of 0.1~10 μg/L and the decisive coefficients(R²)were in the range of 0.9976~0.9997.The average recoveries of spikes samples ranged from 76.9% to 127.7% with relative standard deviations(RSD)of intra- and inter-day in the ranges of 1.0%~9.5% and 2.1%~8.7%,respectively. The limits of detection(LODs)and quantification(LOQs)were 0.01~0.1 and 0.025~0.25 μg/kg,respectively. The method has the advantages of short analysis time,high accuracy and good precision,and can meet the requirements of simultaneous detection of various heterocyclic amines in stewed meat.