摘要:
本研究采用Box-Behnken双响应面优化法结合Matlab分析法对葡萄籽多酚(Grape seed polyphenols,GSP)提取工艺进行了优化。通过考察提取介质、溶剂浓度、料液比、提取时间四个单因素对GSP含量及羟自由基清除率的影响,经过双响应面优化及Matlab分析,得到最优提取工艺为:丙酮浓度78.7%,料液比1:45 g/mL,提取时间91.2 min,得到GSP实际值为(75.39±0.10)μg没食子酸当量(gallic acid equivalent,GAE)/mg干重(dry weight,DW),羟自由基清除率为70.82%±0.11%。此外,经matlab分析,当提取时间取中间值(C=90 min)时,GSP提取量(y1)和羟自由基清除率(y2)均能够取得最大理论值;当丙酮浓度处于76%~82%区间,料液比越趋近1:45 mL/g时,y1和y2的值越趋近最大值。双响应面优化与matlab法优化结果具有一致性,两者结合不仅能够准确得到最佳提取方案,也能够更直观地找出合理的工艺参数区间。
Abstract:
In this study,Box-Behnken-double response surface optimization method combined with Matlab analysis method to be used to optimize the grape seed polyphenols(GSP). The influence of four single factors(extracted medium,solvent concentration,solid-liquid ratio,extraction time)on the content and hydroxyl radical scavenging rate of GSP were investigated. By the optimization of dual response surface combined with Matlab analysis,the optimal extraction process parameter was as follows:Acetone concentration of 78.7%,solid-liquid ratio of 1:45 g/mL,extracting time of 91.2 min. On this condition,the actual value of polyphenol extraction amount was(75.39±0.10)μg gallic acid equivalent(GAE)/mg dry weight(DW),and the hydroxyl radical scavenging rate was 70.82%±0.11%. In addition,through Matlab analysis,when the extraction time was taken as the median value(C=90 min),y1 and y2 were both able to obtain the largest theoretical values. When acetone concentration was within the range of 76%~82%,and the closer the solid-liquid ratio was to 1:45 mL/g,the closer the values of y1 and y2 were to the maximum value. The results of dual response surface optimization and Matlab optimization were consistent. The combination of the two methods can not only accurately obtain the best extraction scheme,but also more intuitively find out the reasonable process parameter interval.
下载:
