Abstract: Beer spoilage microorganisms can affect the quality of beer and also cause some safety problems. Therefore,the detection of spoilage microorganisms in brewing process is quite necessary and important. The traditional cultivation method is simple,easy and precise,but it is very time-consuming. With the development of the brewing industry and the molecular biology technology,a number of detection technologies,which are effective and sensitive,have emerged. These technologies include the modified cultivation methods,bioluminescence as well as chemiluminescence method,PCR-based methods,nucleic acid hybridization-based method and maldi-tof mass spectrometry method. In this article,the progresses and the principles on the above mentioned methods were summarized,and their advantages as well as the limitations were disscussed. This review will provide some useful information for the detection of beer spoilage microorganisms in brewing process.