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中国精品科技期刊2020
郑晓琴, 刘英玉, 王春梅, 郑晓风, 朱明月, 胥兰, 姚刚, 彭斌. 应用SE-μPADs法检测库尔勒市动物源食品中沙门菌的效果评价[J]. 食品工业科技, 2019, 40(19): 230-233,239. DOI: 10.13386/j.issn1002-0306.2019.19.039
引用本文: 郑晓琴, 刘英玉, 王春梅, 郑晓风, 朱明月, 胥兰, 姚刚, 彭斌. 应用SE-μPADs法检测库尔勒市动物源食品中沙门菌的效果评价[J]. 食品工业科技, 2019, 40(19): 230-233,239. DOI: 10.13386/j.issn1002-0306.2019.19.039
ZHENG Xiao-qin, LIU Ying-yu, WANG Chun-mei, ZHENG Xiao-feng, ZHU Ming-yue, XU Lan, YAO Gang, PENG Bin. Evaluation of SE-μPADs Method for Detection of Salmonella in Animal Foods of Korla City[J]. Science and Technology of Food Industry, 2019, 40(19): 230-233,239. DOI: 10.13386/j.issn1002-0306.2019.19.039
Citation: ZHENG Xiao-qin, LIU Ying-yu, WANG Chun-mei, ZHENG Xiao-feng, ZHU Ming-yue, XU Lan, YAO Gang, PENG Bin. Evaluation of SE-μPADs Method for Detection of Salmonella in Animal Foods of Korla City[J]. Science and Technology of Food Industry, 2019, 40(19): 230-233,239. DOI: 10.13386/j.issn1002-0306.2019.19.039

应用SE-μPADs法检测库尔勒市动物源食品中沙门菌的效果评价

Evaluation of SE-μPADs Method for Detection of Salmonella in Animal Foods of Korla City

  • 摘要: 目的:为了快速检测动物性食品中沙门菌的污染。方法:应用国家标准方法和SE-μPADs快速检测方法,对库尔勒市场及屠宰场中的380份样品进行沙门菌检测的评价。结果:国家标准方法中选择性培养基的初筛率为20.00%(76/380),从初筛菌株中分离鉴定出6株沙门菌;SE-μPADs快速检测方法的初筛率为6.58%(25/380),最终分离鉴定出10株沙门菌。SE-μPADs快速检测方法与国家标准方法阳性符合率为100%。库尔勒市场及屠宰场中共检测出10株沙门菌,总检出率为2.63%,而肉样污染率最高,胴体拭子次之,污染率分别达3.66%、3.31%。结论:SE-μPADs快速检测方法的初步应用效果较好,适合推广应用,且库尔勒市应加强对屠宰场及市场上肉品中沙门菌的污染监测。

     

    Abstract: Objective:In order to quickly detect Salmonella contamination in animal foods. Methods:Salmonella was detected and evaluated in 380 samples from markets and slaughterhouses in Korla by using the national standard method and the rapid detection method of SE-μPADs. Results:In the national standard method,the initial screening rate of selective medium was 20.00%(76/380). Six Salmonella strains were isolated and identified from the initial screening strains. The initial screening rate of SE-μPADs rapid detection method was 6.58%(25/380). Ten Salmonella strains were finally isolated and identified. The positive coincidence rate was 100% between the SE-μPADs rapid detection method and the national standard method. A total of ten Salmonella strains were detected in Korla market and slaughterhouse. The total detection rate was 2.63%. The contamination rate of meat samples were the highest in the all samples. The carcass swabs were higher in other sample. The samples pollution rate was 3.66% and 3.31%,respective. Conclusion:the preliminary application effect of the rapid detection method of SE-μPADs is preferably and suitable for the application and promotion. The monitoring of Salmonella contamination in meat prducts of slaughterhouses and market should be strengthened in korla.

     

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