Abstract: To study the effects of different cherry cultivars on phenolic acids and its antioxidant activities,free,conjugated,and bound phenolic acids of five cherry cultivars,including Caihong,Sunburst,Summit,23-51,and Valeri,were identified and quantified by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). There was great variation of phenolic acid content among different cultivars. The total phenolic acid content ranged from 115.96 to 704.94 mg/kg FW. The highest content of phenolic acids was found in Valeri,followed by Summit and 23-51,and the least amounts were identified in Sunburst and Caihong. The results indicated that both of the free and conjugated phenolic acids showed high levels in all the five cherry cultivars,while bound-form phenolic acids were detected in relatively small amounts. Neochlorogenic acid was the most abundant free phenolic acids in sweet cherries,and accounted for more than 90% of the total free fractions. Caffeic acid was found to be the major compound in conjugated and bound forms,accounting for over 50% of the total content of their respective types. DPPH radical scavenging and FRAP assays indicated that Valeri contained the highest antioxidant activity,which had a higher consistency to the phenolic contents. The different cherry cultivars were well discriminated according to PLS-DA. The compounds for discrimination were neochlorogenic acid and caffeic acid(VIP score>1),and both of the two compounds showed the highest levels in Valeri. It is suggested that phenolic acid content was related to cherry cultivars,which was also positively related to its antioxidant properties.