Abstract:
The GNM C7-8 real-time fluorescent PCR system was used to simultaneously and rapidly detect various animal-derived ingredients in adulterated meat products. Bovine meal powder, donkey meal powder, ostrich meal powder and ovine meal powder were respectively mixed with two or three kinds of other animal meal powders of the porcine, duck, horse and chicken. Four groups of artificial adulterated meat products were prepared. They were detected and compared with various animal-derived ingredients by GNM C7-8 real-time fluorescent PCR and ABI 7500 fluorescent PCR. The results showed that the number of cycles detected by GNM C7-8 real-time fluorescence PCR for five kinds of ingredients of pigs, ducks, horses, chickens and foxes from the adulterated meat products was the same as that detected by ABI7500 fluorescent PCR method and was 31, 31, 29, 32 and 25 respectively. However, GNM C7-8 real-time fluorescent PCR based on eight modules could simultaneously detect different animal-derived components in four adulterated meat products, and the detection time was shorter. Therefore, the eight modules of GNM C7-8 real-time fluorescent PCR system could run independently without interference and cross-contamination and realize simultaneously and rapidly detection of various animal-derived ingredients in adulterated meat products, which would provide powerful method support for the detection of adulterated meat products.