摘要:
以青海枸杞蜜为研究对象,在测定其总酚、总黄酮含量和抗氧化活性基础上,采用彗星电泳技术,研究了枸杞蜜对羟基自由基诱导的小鼠淋巴细胞DNA氧化损伤的保护作用。结果表明,枸杞蜜不仅能够有效地清除自由基,而且具有较强的抗氧化活性,枸杞蜜的抗氧化活性与其总酚含量有关(p<0.05),Fe2+络合力与其总黄酮含量相关(p<0.01)。彗星电泳结果表明,枸杞蜜能够显著降低羟基自由基诱导的小鼠淋巴细胞DNA的氧化损伤,与模型组相比,加入枸杞蜜的保护组彗星尾部DNA比例、尾矩(TM)和Olive尾矩(OTM)显著降低(p<0.05),且与枸杞蜜浓度存在明显的剂量-效应关系,线性回归方程决定系数依次为:R2=0.9775,R2=0.9341,R2=0.9425。本文的研究结果将为枸杞蜜功能性食品的开发利用提供依据。
Abstract:
To study antioxidant activity of medlar honey,the total phenolic contents,total flavonoids contents and antioxidant activity in vitro of medlar honey from Qinghai province were determined. Protective effect of medlar honey on the oxidative damage of DNA induced by hydroxyl radicals was examined by comet electrophoresis. The results showed that medlar honey possessed capability to effectively remove free radicals and strong antioxidant activities. The antioxidant activity of medlar honey was related to its total phenol contents(p<0.05),while the ferrous ion-chelating activity of medlar honey correlated with it’s total flavonoids contents. The results of comet electrophoresis showed that the DNA oxidant damage of mice lymphocytes induced by hydroxyl radicals was prevented by medlar honey. Compared to model group,the percent of DNA in the comet tail,tail moment and olive tail moment of experience group all significantly reduced(p<0.05)in a dose-dependent manner. The determinant coefficient of linear regression equation between the percent of DNA in the comet tail,tail moment,olive tail moment and concentration of medlar honey were R2=0.9775,R2=0.9341 and R2=0.9425,respectively. This study would provide a basis for the development and utilization of medlar honey as functional food.
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