表面增强拉曼光谱测定保健品中非法添加物西地那非

吴国萍; 周亚红; 李静泉; 李家伟

doi:10.13386/j.issn1002-0306.2019.11.042

表面增强拉曼光谱测定保健品中非法添加物西地那非

1. 江苏警官学院刑事技术系, 江苏南京 210031);
2. 江苏省食品药品与环境犯罪技术检验工程实验室, 江苏南京 210031

基金项目:

江苏警官学院科研创新团队(2018SJYTD03)

公安部软课题项目(2016LLYJJSST025)

"十三五"江苏省重点建设学科建设工程。

江苏省教育厅自然科学重大项目(18KJA620001)

详细信息

作者简介:
吴国萍(1972-),女,博士,教授,研究方向:毒物、毒品与微量物证分析,E-mail:wuguoping@jspi.cn。

中图分类号: TS201.1
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出版历程
- 收稿日期: 2018-08-26
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-05-31

Surface Enhanced Raman Spectroscopy(SERS) for the Detection of Sildenafil in Health Care Products

1. Department of Criminal Science and Technology, Jiangsu Police Institute, Nanjing 210031, China);
2. Jiangsu Engineering Laboratory of Food, Drug, and Environment Criminal Investigation and Forensic Analysis, Nanjing 210031, China

摘要

摘要: 基于表面增强拉曼光谱技术(SERS),采用自制表面增强试剂(纳米金)与厂家提供的表面增强试剂,对比研究了对21种查缴的保健品样品中的非法添加物西地那非的快速拉曼检测效果,同时考察了不同配比纳米金和不同浓度的盐酸、硝酸和硫酸作为助剂的增强效果。结果表明,自制纳米金表面增强试剂(氯金酸和柠檬酸钠的体积比1:1)及2.67 mol·L^-1的硝酸表面增强效果为佳,可对浓度为0.01 μg·mL^-1西地那非阳性检出;与飞行时间-液质联用仪(QTOF-LC-MS)分析结果对比,自制纳米金检测吻合度为100%,厂家提供的增强试剂检测吻合度为86.4%。综上,自制表面增强试剂(纳米金)表面增强效果不差于厂家提供的表面增强试剂。
- 表面增强拉曼光谱 /
- 保健品纳米金 /
- 西地那非
Abstract: Based on surface-enhanced raman spectroscopy(SERS),the illegal additive sildenafil in 21 samples of seized health care products was detected by self-made nano-gold as the surface-enhanced reagents comparing with those provided by the manufacturers. The enhancement effects of nano-gold in various proportions with using diverse concentrations of hydrochloric acid,nitric acid and sulfuric acid were additionally measured. The results showed that:As the minimum detection limit of sildenafil was 0.01 μg·mL^-1,the enhancement effect of self-made nano-gold as the surface-enhanced reagent(the volume of cholor-gold acid and sodium citrate was in the proportion 1:1)added nitric acid with the concentration of 2.67 mol·L^-1 was as result the best among the all tests,so that it was 100% consistent with the results analyzed by QTOF-LC-MS,while those provided by the manufacturer were only 86.4% consistent. This indicated that the surface enhancement effect of the self-made surface enhancement reagent(nano-gold)was not inferior to that provided by the manufacturer.
- surface enhanced Raman spectroscopy /
- health care products /
- nano-gold