Abstract: Using Termitomyces albuminsous as experimental materials,the optimum conditions of ultrasonic-assisted extraction from Termitomyces albuminsous with the extraction rate of protein as index were studied through the method of single factor and orthogonal experiments. The crude protein’s antioxidant activities of ABTS⁺·,DPPH· and O₂^-· radical scavenging assays were also investigated in this paper.The results showed that the optimum extraction conditions were ultrasonic power 400 W,ultrasonic temperature 70 ℃,ultrasonic time 1.5 h,pH11 and solid-to-solvent ratio 1:25 g/mL,respectively. The extraction rate of crude protein could reach up to 67.322%±1.028% and the content of protein was 86.525%±1.315% under these conditions. Termitomyces albuminosus crude protein had a certain antioxidant activity. The scavenging ability of ABTS⁺·,DPPH· and O₂^-· free radical IC₅₀(half maximal inhibitory concentration)value were separately 46.322,61.176 and 151.250 mg/L. The free radical scavenging capacity of Termitomyces albuminosus protein was lower than that of V_C’s ability to remove them. Therefore,the ultrasonic-assisted technology was suitable for crude protein extraction from Termitomyces albuminosus and the protein had antioxidant activity. The study provided a theoretical basis for high value processing of Termitomyces albuminosus.