Abstract: A strain of high acid-producing(Lactobacillus plantarum T-02)was isolated from tofu acid seriflux of natural fermentation by the plate coating method,and the yield of acid and pH of the system were taken as indicators,in order to clarify the dominant acid-producing strains in natural fermentation syrup and lay the foundation for the industrial production of acid seriflux tofu. The strain was used to prepare fermented yellow seriflux and the results showed that the pH values declined to 3.6 and it could produce 35.3 g/L acidity after fermentation 24 h. And it could produce 40.8 g/L acidity after fermentation 48 h,indicating that it could be used as a pure fermentation strain of acid seriflux bean curd.The strain was identified as Lactobacillus plantarum using morphology combined with physiological and biochemical tests and molecular biology methods.