Abstract: Three yeast strains(Y-1,Y-2 and Y-3)were isolated from the Jiaozi. The yeasts were used to study the differences in the fermentation performance. After the analysis of ITS sequence,Y-1 and Y-2 were identified as Saccharomyces cerevisiae and Y-3 was the Trichosporon asahii. The results showed that the growth of Y-1 and Y-2 were stronger than Y-3,the optimum growth temperature of the three yeast strains were 28,28~32 and 36 ℃,respectively. The optimum growth pH of the three yeast strains were 5.1~5.6,5.1 and 4.1,respectively. Fermentation ability,liquefaction power,saccharification power and esterification power all showed Y-2>Y-1>Y-3.Therefore,through the analysis of the fermentation performance of the three yeast strains,it was found that the performance of Saccharomyces cerevisiae was better than Trichosporon asahii.