Research of Fatty Acid Deposition Mechanism in Adipose Tissue of Sunit Sheep
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摘要: 本文以12月龄苏尼特羊不同脂肪组织(皮下脂肪、尾部脂肪、肾脏脂肪)为试验对象,利用气相色谱-质谱(GC-MS)技术和实时荧光定量PCR技术研究不同脂肪组织中脂肪酸含量和脂肪代谢相关基因mRNA表达量,同时分析脂肪代谢相关基因与脂肪酸间相关性。结果表明,苏尼特羊脂肪组织中脂肪酸以棕榈酸、硬脂酸和油酸为主。肾脂中硬脂酸的含量显著高于尾脂(p<0.05);尾脂中油酸含量显著高于皮脂和肾脂(p<0.05)。不同脂肪组织间,肾脂中硬脂酰辅酶A去饱和酶(SCD)、脂蛋白脂肪酶(LPL)和脂肪酸合成酶(FASN)基因mRNA表达量显著高于皮脂和尾脂(p<0.05);皮脂中肉碱棕榈酰转移酶(CPT1)和乙酰辅酶A羧化酶(ACC)基因mRNA表达量显著高于肾脂(p<0.05)。通过相关性分析,共轭亚油酸与FADS1、LPL和SCD呈显著正相关(p<0.05);二十碳五烯酸(C20:5)与FADS1和LPL间呈显著正相关(p<0.05),与ACC呈显著负相关(p<0.05)。由此可知,调控脂肪酸沉积相关基因的表达对脂肪酸沉积的方向和部位有明显的影响。Abstract: The different adipose tissue(subcutaneous fat,tail fat and kidney fat)of Sunit sheep with 12 months week age was used as the test object,and the gas chromatography-mass spectrometry(GC-MS)and real time polymerase chain reaction(RT-PCR)were used to research the contents of fatty acid and the mRNA expression of fat metabolism related gene,and the relationship between fat metabolism gene and fatty acid were also analyzed. The results showed that fatty acid in adipose tissue of Sunit sheep mainly included palmitic acid,stearic acid and oleic acid. The contents of stearic acid in kidney fat was significantly higher than that in tail fat(p<0.05),the ratio of oleic acid in tail fat was significantly higher than that in subcutaneous fat and kidney fat(p<0.05). The gene expression of FASN,SCD and LPL in kidney fat was significantly higher than that in subcutaneous fat and tail fat(p<0.05),the gene expression of CPT1 and ACC in subcutaneous fat was significantly higher than that in kidney fat(p<0.05). Correlation analysis showed that CLA was positively correlated with FADS1,LPL and SCD(p<0.05),C20:5 was positively correlated with FADS1 and LPL(p<0.05)and negatively correlated with ACC(p<0.05). In conclusion,regulating the expression of genes related to fat deposition had a significant effect on the direction and location of fatty acid deposition.
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Keywords:
- Sunit sheep /
- adipose tissue /
- fatty acid /
- fat metabolism
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