Abstract: Taking Lactobacillus plantarum HNU082 isolated from traditional fermented Yucha of Hainan, the antibiotic resistance gene information carried by the strain was interpreted by whole genome sequencing (arlR, emeA, fexA, lmrC, lmrD, macB, mepA, mprF, novA, pbp1a, gyrB, and rpoB). Based on this, resistance screening was carried out for 11 commonly used antibiotics. Thus the minimum inhibitory concentrations (MIC) of the 11 antibiotics against HNU082 were obtained to determine the relationship between the resistance gene and antibiotic resistance under specific experimental conditions. The results showed that not all resistance genes could cause strains to develop resistance to the corresponding antibiotics. For example, rpoB was detected from the strain, but didn't express rifampicin resistance. The degree of resistance was independent on the proportion of resistance genes. Vancomycin and norfloxacin-related genes respectively accounted for 6.7% and 20.0% of the total detected genes, but the resistance of strains to both genes was MIC>1280 μg/mL. While clindamycin hydrochloride-related genes accounted for 20.0% of the detected genes, the MIC (20 μg/mL) was quite different from that of norfloxacin. The related resistance genes of erythromycin and roxithromycin were macB, but the resistance of the strain to roxithromycin was stronger than that of erythromycin. So different drugs affected by the same gene would have different resistance characteristics.