Abstract: This article aimed to screen endophytic bacteria with broad-spectrum antibacterial activity and investigate the post-harvest preservation effect of their fermentation products on cherry cherries. Eighteen endophytic bacteria were isolated and purified from the roots, stems, leaves or fruits of healthy kiwifruits by grinding method. The strong active strain MR-1 was selected by plate sputum method, and the bacteria, colony morphology, physiological and biochemical determination 16S rRNA sequencing analysis and strain identification. The inhibitory effect of the active strain on eight common pathogenic bacteria and the inhibitory effect of the main pathogen of cherry sage were further studied. The results showed that the strain MR-1 had strong antagonistic effect on postharvest pathogenic bacteria, and it was initially identified as Bacillus amyloliquefaciens, which had strong antagonistic effect on 8 plant pathogens. It showed strong inhibitory effects on Alternaria nees, Ascomycota and Colletotrichum capsici three main disease bacterium. Cherry cherries treated with endophytic MR-1 fermentation filtrate had the best preservation effect, and the compound fruit and vegetable preservatives followed. On the 8th day of storage, comparing with the blank control group, the decay diameter of MR-1 group decreased by 50.6%, 50.6% and 44.9%. The secondary metabolites of Bacillus amyloliquefaciens MR-1 were abundant, which had a good application prospect in the preservation of fruits and vegetables.