Abstract: In order to investigate the effect of 1-MCP combined with Trichoderma harzianum on the storage quality of cherry tomato,the physiological index,nutrition index and related enzyme activities of cherry tomato were determined with"honor F1 generation"cherry tomato as the material,and the effects of four dealing methods (preharvest distilled water spray+postharvest distilled water treatments (S1),preharvest 3.0×10⁶ CFU/mL Trichoderma harzianum spray+postharvest distilled water treatments (S2),preharvest distilled water spray+postharvest 0.5 μL/L 1-MCP treatments (S3),preharvest 3.0×10⁶ CFU/mL Trichoderma harzianum spray+postharvest 0.5 μL/L 1-MCP treatments (S4) on the storage quality of cherry tomato at (11±0.5)℃ were studied. The results showed that,Compared with the control (S1),the three treatments could inhibit the increase of fruit decay rate and delay the decrease of fruit firmness,soluble solids,titratable acid content and V_C content. In addition,it could also reduce the respiration rate and ethylene production rate of fruit,maintain SOD activity,PPO activity,POD activity and LOX activity well. Among them,the decay rates of S1 (CK),S2 (Trichoderma harzianum),S3 (1-MCP) and S4 (Trichoderma harzianum with 1-MCP) were 49.85%,37.89%,28.56% and 19.81% at the end of storage (48 d),respectively. By comparison,the combination of spraying 3.0×10⁶ CFU/mL Trichoderma harzianum before picking and treating cherry tomato with 0.5 μL/L 1-MCP after picking was the best for fruit storage,which could significantly delay the senescence process of cherry tomato and maintain high storage quality. Therefore,the combination of spraying 3.0×10⁶ CFU/mL Trichoderma harzianum before picking and treating cherry tomato with 0.5 μL/L 1-MCP after picking had the best fresh-keeping effect.