婴幼儿配方乳粉加工环境中蜡样芽孢杆菌多位点序列分型

吴霜; 张紫薇; 李虹甫; 孙露宏; 王蕊; 满朝新; 郭鸰; 姜毓君

doi:10.13386/j.issn1002-0306.2018.23.024

婴幼儿配方乳粉加工环境中蜡样芽孢杆菌多位点序列分型

Multilocus Sequence Typing of Bacillus cereus Isolates from the Processing Environment of Powdered Infant Formula

摘要

摘要: 以分离自婴幼儿配方乳粉加工环境中蜡样芽孢杆菌为研究对象,利用多位点序列分型(MLST)技术对蜡样芽孢杆菌的多样性和系统进化关系进行探究。结果表明,84株蜡样芽孢杆菌共分成24个ST型,分别是ST-24、ST-26、ST-32、ST-62、ST-144、ST-374、ST-999、ST-1119、ST-1243、ST-1284、ST-1333、ST-1334、ST-1335、ST-1336、ST-1337、ST-1338、ST-1339、ST-1340、ST-1341、ST-1342、ST-1343、ST-1344、ST-1345及ST-1347,其中ST-999(22.62%)、ST-1343(15.48%)、ST-1335(7.14%)与ST-1345(7.14%)是该婴幼儿配方乳粉加工环境中的优势ST型。同时发现了3个新的等位基因pur-242,pyc-198,ilv-276与14个新的ST型ST-1333、ST-1334、ST-1335、ST-1336、ST-1337、ST-1338、ST-1339、ST-1340、ST-1341、ST-1342、ST-1343、ST-1344、ST-1345和ST-1347。系统发育分析表明24个ST型与B.cereus、B.anthracis和B.thuringiensis这三个种显示了更近的系统发育关系,与蜡样芽孢杆菌群体中的另外8个种的亲缘关系较远。

Abstract: Multilocus sequence typing(MLST)was used to analyze the diversity and phylogenetic relationship of Bacillus cereus strains isolated from the processing environment of infant formula milk powder. The results indicated that 84 Bacillus cereus strains were divided into 24 sequence types(ST),including ST-24,ST-26,ST-32,ST-62,ST-144,ST-374,ST-999,ST-1119,ST-1243,ST-1284,ST-1333,ST-1334,ST-1335,ST-1336,ST-1337,ST-1338,ST-1339,ST-1340,ST-1341,ST-1342,ST-1343,ST-1344,ST-1345 and ST-1347. ST-999(22.62%),ST-1343(15.48%),ST-1335(7.14%)and ST-1345(7.14%)were the predominant STs. Three new alleles,pur-242,pyc-198,ilv-276 and 14 new STs,ST-1333,ST-1334,ST-1335,ST-1336,ST-1337,ST-1338,ST-1339,ST-1340,ST-1341,ST-1342,ST-1343,ST-1344,ST-1345 and ST-1347,were found in this study. Phylogenetic analysis revealed the diversity of Bacillus cereus group from the processing environment and showed that 24 STs were close to B. cereus,B. anthracis and B. thuringiensis,and they were far from the other eight species.

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