Antioxidant and Antibacterial Abilities of Crude Peptides Extracted from the Three Chinese Traditional Dry-Cured Hams
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摘要: 提取宣威火腿、金华火腿、如皋火腿中粗多肽并比较其体外抗氧化活性与抑菌活性。分别以三种干腌火腿为材料提取粗多肽,测定火腿中股二头肌部分的盐含量及火腿中粗多肽的肽含量。测定粗肽液清除DPPH自由基能力、铁离子还原能力、清除ABTS+自由基能力和氧自由基吸收能力并分析肽中氨基酸成分,比较三种火腿中粗多肽生物活性的差异。结果表明,宣威火腿盐含量显著低于其他两种火腿(p<0.05),粗多肽的肽含量显著高于如皋火腿(p<0.05);三种火腿粗多肽中都富含Glu和Asp,宣威火腿粗多肽中Val和Pro等疏水性氨基酸的含量显著高于其他两种火腿(p<0.05)。在质量浓度1.0、5.0 mg/mL时,宣威火腿粗肽液清除DPPH自由基能力最强;宣威火腿粗肽液铁离子还原能力和氧自由基吸收能力显著高于金华火腿及如皋火腿粗肽液(p<0.05);三种干腌火腿都具有较强的抑制大肠杆菌的能力,在质量浓度大于0.5 mg·mL-1时,宣威火腿与如皋火腿粗肽液抑制大肠杆菌能力显著大于金华火腿(p<0.05),抑菌率接近50%。与其它两种火腿粗多肽比较,宣威火腿粗肽中含有较高的肽含量和较多的疏水性氨基酸,宣威火腿粗肽液具有更高的抗氧化能力与抑菌能力。Abstract: The aim of this study was to compare the vitro antioxidant and antibacterial abilities of crude peptides extracted from Xuanwei ham(XH),Jinhua ham(JH)and Rugao ham(RH). Crude peptides were collected from XH,JH and RH. The content and composition of amino acid and the antioxidant and antibacterial activities of crude peptides were measured. The antioxidant abilities of different crude peptide extracts were determined by DPPH radical scavenging ability,ferric reducing antioxidant power(FRAP),ABTS+·scavenging ability(ABTS)and oxygen radical absorbance capacity(ORAC). Results showed that,the content of NaCl of XH was significantly lower than JH and RH(p<0.05).The contents of crude peptides extracted from XH(XHP)were significantly higher than those from RH(RHP)(p<0.05). Amino acid analysis showed that XHP,JHP and RHP all contained abundant Pro and Glu. XHP owned higher content of hydrophobic amino acids including Val and Pro than JHP and RHP(p<0.05). At the concentration of 1.0 and 5.0 mg/mL,XHP presented higher DPPH radical scavenging activity than JHP and RHP(p<0.05). XHP possessed stronger FRAP and ORAC than JHP and RHP(p<0.05). All of three kinds of crude peptide presented strong antibacterial ability. When the concentration was above 0.5 mg/mL,antibacterial abilities of XHP and RHP were significantly higher than JHP(p<0.05),and the antibacterial rates of XHP and RHP were approximately 50 percent. XHP possessed stronger bioactivities including antioxidant and antibacterial abilities in comparison to JHP and RHP. XHP had higher content of peptides and more abundant hydrophobic amino acids which might contribute to its stronger antioxidant and antibacterial capacities than JHP and RHP.
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Keywords:
- Xuanwei ham /
- Jinhua ham /
- Rugao ham /
- antioxidant activity /
- antibacterial activity
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