Abstract: To further enhance the use of Pleurotus nebrodensis proteins(FPs),the exhalation rate and physicochemical properties of proteins were separated out when the percentage base saturation was equal to 60%(F60),80%(F80),90%(F90),which were named FPs collectively. And the precipitation rate,physicochemical properties of FPs and the functional properties of F60 were determined. The results showed the exhalation rate of F60(65.62%)was higher than F80 and F90. No significant difference of the content of disulfide bond was found between FPs(p>0.05). FPs had one band(at about 31 kDa)and the protein bands of FPs existed differences. F60 exhibited higher surface hydrophobicity and fluorescence intensity,and included more β-sheet than F80 and F90. F60 exhibited good solubilities,foaming capacities,and excellent in-vitro digestibilities. Pleurotus nebrodensis proteins is a kind of high grade protein resource.