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中国精品科技期刊2020
王文宁, 张晓峰, 韩萍, 于斐, 曹阳. 蔓菁对小鼠肠道菌群的影响[J]. 食品工业科技, 2018, 39(14): 287-291. DOI: 10.13386/j.issn1002-0306.2018.14.054
引用本文: 王文宁, 张晓峰, 韩萍, 于斐, 曹阳. 蔓菁对小鼠肠道菌群的影响[J]. 食品工业科技, 2018, 39(14): 287-291. DOI: 10.13386/j.issn1002-0306.2018.14.054
WANG Wen-ning, ZHANG Xiao-feng, HAN Ping, YU Fei, CAO Yang. Effects of Turnip on Intestinal Flora of Mice[J]. Science and Technology of Food Industry, 2018, 39(14): 287-291. DOI: 10.13386/j.issn1002-0306.2018.14.054
Citation: WANG Wen-ning, ZHANG Xiao-feng, HAN Ping, YU Fei, CAO Yang. Effects of Turnip on Intestinal Flora of Mice[J]. Science and Technology of Food Industry, 2018, 39(14): 287-291. DOI: 10.13386/j.issn1002-0306.2018.14.054

蔓菁对小鼠肠道菌群的影响

Effects of Turnip on Intestinal Flora of Mice

  • 摘要: 目的:探究不同剂量蔓菁对小鼠粪样中肠道菌群和短链脂肪酸的影响。方法:选取BalB/C雄性小鼠(18~22 g)48只按体重随机分为对照组、低剂量组、中剂量组、高剂量组4组,三个剂量组分别饲喂不同剂量(1、2、4 g/kg·BW·d)的蔓菁粉,对照组正常喂养,连续干预14 d,分别在干预第0 d、第7 d、第14 d称重并无菌收集新鲜粪样。采用平板计数法检测肠道菌群数量,气相色谱-质谱联用法检测短链脂肪酸的含量,并用SPSS 21.0对结果进行数据分析。结果:蔓菁可增加肠道益生菌乳酸杆菌、双歧杆菌和中性菌肠杆菌的数量(p<0.05),减少中性菌肠球菌和致病菌产气荚膜梭菌的数量(p<0.05),中剂量(2 g/kg·BW·d)作用最为明显,且肠杆菌数量增加幅度低于乳酸杆菌和双歧杆菌增加幅度。蔓菁还可增加肠道乙酸、丙酸、正丁酸、异丁酸的含量(p<0.05),提高短链脂肪酸总含量,高剂量(4 g/kg·BW·d)作用最为明显。结论:蔓菁可促进小鼠肠道有益菌的增殖,抑制致病菌的增殖;蔓菁可促进小鼠肠道短链脂肪酸的产生。

     

    Abstract: Objective:To explore the effects of turnip of different concentrations on intestinal flora and short-chain fatty acids in the feces of mice. Methods:48 BalB/C male mice(18~22 g)were randomly assigned to control group,low-dose group,middle-dose group and high-dose group. The three dose groups were fed with turnip of different doses(1,2,4 g/kg·BW·d),while the control group was fed normally for 14 days. The mice were weighed and feces were collected for the determination of the number of intestinal flora and the content of short-chain fatty acids at 0,7,14 days. The number of intestinal flora and the content of short-chain fatty acids was detected by plate counting method and gas chromatography-mass spectrometry,and the results were analyzed by SPSS 21.0. Results:Turnip could increase the number of Lactobacillus,Bifidobacterium,and Enterobacteriaceae(p<0.05),while decrease the number of Enterococcus and Clostridium perfringens(p<0.05),and the effect of middle dose(2 g/kg·BW·d)was obviously. In addition,the increase rate of Enterobacteriaceae was lower than that of Lactobacillus and Bifidobacterium(p<0.05). Turnip could promote the production of acetic acid,propionic acid,n-butyric acid,isobutyric acid,so as to improve the contents of short chain fatty acids,and the high dose(4 g/kg·BW·d)was the most significant(p<0.05). Conclusion:Turnip could promote the proliferation of beneficial bacteria and inhibit the proliferation of pathogens in mice and promote the production of short chain fatty acids in mice.

     

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