Abstract: The plant polysaccharide has lots of bioactive functions such as antioxidation and other biological activities. At present,various plant polysaccharide was extracted successfully and used widely in the research of nutrition food and medicine. This article summarized different extraction methods of the plant polysaccharide(hot dipping extraction,acid-alkali extraction,ultrasonic extraction,microwave extraction,CO₂ supercritical fluid extraction,ultrafiltration extraction),and analyzed the advantages,disadvantages and influence factors of different methods. Aimed at the disturb of protein in the polysaccharide extraction,it compared three common methods of deproteinization:TCA method,Hydrochloric acid method and Sevage method. Human's antioxidant system is similar to the immune system with thorough and complex function,stronger antioxidant ability. Antioxidant is an important step in the body's own defense,because the free radicals or antioxidants will break down cells and tissues,which affects metabolic function,and lead to a series of health problems. Antioxidant can delay exercise-induced fatigue and accelerate physical recovery,therefore,the researches aiming at antioxidant properties of plant polysaccharide become a hotspot in food science and medicine research in recent years.