Abstract: Tilapia(GIFT,O.niloticus♀╳O.aureus♂)cultured under monoculture mode,polyculture mode with shrimp,polyculture mode with freshwater fish from southern China were collected. The contents of basic nutritional compositions,amino acid and fatty acid in their muscle were determined and the differences were analyzed from species,breeding modes and cultured areas. Results showed that there had little difference between GIFT and O.niloticus♀╳O.aureus♂ tilapia of their basic nutrients and fatty acid,but higher contents of total amino acid(19.51 g/100 g)and essential amino acid(7.96 g/100 g)were observed in the latter(p<0.05). Between the two polyculture mode,several kinds of flavoraminoacid,essential amino acidandnon-essential amino acid content were higher in tilapia fed with freshwater fish(p<0.05),but no significant difference was observed when compared with the monoculture mode. Among the four cultured areas,tilapia from Guangdong Province contain higher amino acid and fatty acid,their flavor amino acid content(7.65 g/100 g)were significantly higher than tilapia from Guangxi and Hainan Province(p<0.05). Oleic acid(1.44 mg/g),linoleic acid(1.02 mg/g)and DHA content(0.91 mg/g)of tilapia from Guangdong Province were higher than tilapia from Guangxi and Fujian Province(p<0.05),higher total amino acid and unsaturated fatty acid content were also observed. In summary,the quality of O.niloticus♀╳O.aureus♂ tilapia quality were better than GIFT,tilapia polyculture with freshwater fish were better than polyculture with shrimp,GIFT tilapia from Guangdong were surpass than others.