Abstract: Objective:Medium was optimized for the production of fumagillin from Aspergillus fumigatus CEA-1701.Method:The content of fumagillin was determined by HPLC. The carbon and nitrogen sources were optimized using single factor experiment dessign. The major components in the medium were further optimized by Plackett-Burman (PB) and Box-Behnken Design (BBD). Results:Results show that the suitable sources of carbon and nitrogen for fumagillin productions are glycerol and yeast extract respectively;Glycerol, corn flour and yeast extract are the main factors affecting the production of fumagillin. BBD test showed that the optimum medium was composed of glycerol 33.5 g/L, yeast extract powder 3.1 g/L, corn flour 1.7 g/L, MgSO₄·7H₂O 0.5 g/L, K₂HPO₄ 1.5 g/L, KCl 0.5·g/L, FeSO₄·7H₂O 0.013 g/L, which resulted in the fumagillin productivity of 68.51 mg/L that was 122.4% higher than that of the initial medium. Conclusion:After the medium optimizition, the fumagillin production significantly increased, which provides a reference for fumagillin production.