Abstract: The coconut oil liposomes were prepared by film dispersion method. Soybean lecithin and hydrogenated soybean lecithin were used as membrane material, and the entrapment efficiency was the main evaluation index. Based on one-factor-at-a-time experiments, orthogonal experimental design was applied to optimize process conditions for the liposomes of the coconut oil. The optimal process conditions were determined as follows:carboxymethyl chitosan and lecithin ratio of 1:16, lecithin and cholesterol ratio of 8:1, coconut oil and lecithin ratio of 3:1, pH4.5, 750 W ultrasonic power and 20 min encapsulation time. Under the optimal conditions, the observed value of encapsulation rate was 96.94%±0.20%.The final product carboxymethyl chitosan coated coconut oil liposomes in 0.9% sodium chloride, 24 h release was 41.28%±0.30%. The encapsulation efficiency and cumulative release of coconut oil liposomes are high, stability is good. It can achieve delayed release and targeted administration.