Abstract: In order to protect the activity of Lactobacillus plantarum to enhance the probiotic function of Lactobacillus in animal intestine,with Lactobacillus plantarum in natural fermented corn silage as the core material and whey protein and gelatin as the wall material,by spray drying technique were used to make the microcapsules.The survival rate of Lactobacillus plantarum was determined as response value,4 factors (i.e., the ratio of the wall, solid content, inlet air temperature, and feed rate) were selected as the response surface to optimize the conditions.The results showed that the optimal conditions were as follows:1:2 ratio of wall material(whey protein:gelatin),22% solid content of wall material, inlet air temperature of 127℃ and feed amount of 35%.Under these conditions,Lactobacillus plantarum embedding rate was 62.15%.In conclusion,this study laid the foundation for the preparation of Lactobacillus plantarum microcapsules by spray drying method.