Abstract: The ADR1 gene of Saccharomyces cerevisiae is a positive regulator of transcription of genes encoding peroxisomal proteins.To regulate alcohol metabolism,it has a positive effect.The full-length sequence of ADR1 gene was cloned from the genomic DNA of Saccharomyces bayanus using PCR for the first time.The ADR1 gene of S. bayanus was cloned,and the physical and chemical properties of ADR1 protein were analyzed by online analytical tools, such as ProtParam, ProtScale, TMHMM, PredictProtein,and Swiss-Model. At the same time, the protein secondary structure and tertiary structures of protein were predicted.The results indicated that the ADR1 gene contained an opening reading frame (ORF) of 3960 bp encoding 1319 predicted amino acids.The protein was a hydrophilic protein exercising their control functions in the nucleus.It contained 17 serine kinase potential phosphorylation sites,4 coil domains,and two zinc finger domains.The protein encoded by ADR1 gene of S.cerevisiae was extremely similar to the predicted protein. It inferred that ADR1 gene of S. bayanus might play a role in regulating alcohol dehydrogenase gene.