Abstract: The effects of compound ratio,solution concentration,cooling process and reaction time on the scavenging ability of DPPH free radical and hydroxyl radical by polysaccharides and flavonoids from Auricularia auricula were studied using ultrasonic extraction with oxygen resistant. The results showed that the content of polysaccharides and flavonoids from Auricularia auricula were 3.85% and 4.2 mg/100 g respectively,and the antioxidant ability of the products was higher than antioxidants from aerobic ultrasonic extraction(p<0.05).The optimal compound ratio of polysaccharides and flavonoids from Auricularia auricula was 7:3,and the DPPH free radical and hydroxyl radical scavenging capacity of mixture were 95% and 62% respectively.The significant positive correlation existed in solution concentration, reaction temperature and DPPH free radical scavenging ability(p<0.05),and in solution concentration,reaction temperature and hydroxyl radical scavenging ability of Auricularia auricula anthocyanins and polysaccharides complex (p<0.05). The polysaccharides and flavonoid from Auricularia auricula had synergistic antioxidant to increase scavenging efficiency of DPPH free radical and hydroxyl radical.