Abstract: The aim of this study was to investigate the protective effects of olive leaf methanolic extract(OLME)on acute liver injury induced by D-galactosamine/lipopolysaccharide(LPS)in mice. Methods:Kunming mice were randomly divided into six group:normal group,model group,positive group(bifendate 200 mg·kg^-1),and OLME at a dose of 200,400,and 800 mg·kg^-1(different experimental groups). The drug was administered once a day for consecutively 14 d. The acute liver injury model was induced by intraperitoneal injection of 600 mg·kg^-1 D-galactosamine and 40 μg·kg^-1 LPS within one hour after last administration,the normal group receiving the same volume of saline. Twelve hours after the last administration,blood and tissues were collected from each mouse. Serum ALT and AST levels were measured by biochemical method. The activities of SOD,GSH-Px,caspase-3 and caspase-8 and the contents of MDA,TNF-α,IL-1β,and IL-6 in liver tissue were measured using kits. Results:Being Compared with the model group,OLME(400 and 800 mg·kg^-1)treatment significantly reduced serum ALT and AST activities(p<0.05),reduced MDA contents in liver homogenate,and increased SOD activity(p<0.05). OLME at 400 and 800 mg·kg^-1 dose groups significantly decreased TNF-α,IL-1β and IL-6 contents(p<0.05). However,the improved effect of OLME on GSH-Px was not obvious,only high OLME dose had certain effect. OLME at 200,400 and 800 mg·kg^-1 dose groups significantly decreased caspase-3 and caspase-8 activities(p<0.05). Conclusion:OLME plays good protective effects to mice with acute liver injury induced by intraperitoneal injection of D-galactosamine and LPS.